Microbiology Research Frontiers

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Research Articles

A Practical Guide to MIQE Guidelines for Diagnostic qPCR: Ensuring Reliable and Reproducible Assay Validation

This comprehensive guide details the application of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines specifically for diagnostic qPCR assay validation.

Michael Long
Jan 12, 2026

The Complete Guide to MIQE Guidelines: Designing and Validating Robust qPCR Assays for Clinical and Biomedical Research

This comprehensive guide details the application of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines for assay design and validation.

Amelia Ward
Jan 12, 2026

MIQE Guidelines in Clinical Chemistry: Implementing Rigorous Standards for Reproducible Biomarker Research and Diagnostic Assay Development

This article provides a comprehensive guide to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, explicitly tailored for clinical chemistry applications.

Mia Campbell
Jan 12, 2026

MIQE Guidelines in qPCR: The Critical Difference Between Publishable and Questionable Data

This article explores the profound impact of MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) compliance on data integrity in biomedical research.

Henry Price
Jan 12, 2026

MIQE-Compliant Primer-Probe Sequences: Essential Disclosure for Reproducible qPCR in Biomedical Research

This article provides a comprehensive guide to MIQE-compliant primer and probe sequence disclosure for quantitative PCR (qPCR) and digital PCR (dPCR).

Joshua Mitchell
Jan 12, 2026

MIQE-Compliant RNA QC for qPCR: The Complete Guide to Accurate Gene Expression Analysis

Accurate quantitative PCR (qPCR) begins with high-quality RNA.

Logan Murphy
Jan 12, 2026

MIQE 2.0 2025 Update: The Essential Guide to Reproducible qPCR for Biomedical Research

This article provides a comprehensive summary of the 2025 update to the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines.

Bella Sanders
Jan 12, 2026

Validating MALDI-TOF MS for Clinical Diagnostics: A Complete Guide for Microbiology Laboratory Implementation

This comprehensive article explores the critical validation process for implementing Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in clinical microbiology laboratories.

Easton Henderson
Jan 12, 2026

Mastering Long-Range PCR: A Comprehensive Protocol for High-Fidelity Genomic DNA Amplification in Research

This detailed guide provides researchers, scientists, and drug development professionals with a complete framework for successful long-range PCR amplification of genomic DNA.

Hannah Simmons
Jan 12, 2026

PCR Detection Limits: A Comparative Guide to Sensitivity Across Methods (2024)

This technical article provides a comprehensive comparison of the limit of detection (LOD) for modern PCR methodologies, including endpoint PCR, quantitative PCR (qPCR), and digital PCR (dPCR).

Grace Richardson
Jan 12, 2026

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